亚洲欧美天堂中文字幕
<sup id="oiuk6"></sup>
<rt id="oiuk6"></rt>
收藏本站    您好,歡迎光臨上海懋康生物科技有限公司!
登錄  |   注冊
當前位置:首頁> 新聞資訊> 精品資訊 >Fluo-3, AM, Cell Permeant 鈣離子熒光探針,超級純
Fluo-3, AM, Cell Permeant 鈣離子熒光探針,超級純
時間:2016-07-02     作者:懋康生物     文章來源:原創    

Fluo-3, AM, Cell Permeant 鈣離子熒光探針,超級純

n   最常用的Ca2+綠色熒光探針

搜索關鍵詞Fluo-3 AM, Cell Permeant 鈣離子熒光探針,超級純;Fluo-3, Acetoxymethyl Ester; 可見光激發波長Ca2+熒光探針;Fluo-4;Fluo-8, AM;Rhod-2, AM;CAS NO121714-22-5;

Fluo-3是一種常用的可見光激發波長Ca2+熒光探針,具有較高的Ca2+捕獲能力,通過熒光強度的變化直接反映Ca2+信號的動力學變化。當Fluo-3以游離配體形式存在時幾乎是非熒光性的,但一旦與Ca2+結合即可產生很強的熒光,熒光信號增強60~80倍,此時的最大激發波長為506nm,最大發射波長為526nm??赏ㄟ^激光共聚焦顯微鏡或流式細胞儀來檢測胞內鈣離子水平的變化。Fluo-3, AMFluo-3的一種乙酰甲酯衍生物,具有細胞膜滲透性,只需簡單培養,即可輕易進入細胞。一旦進入細胞內,即被其內酯酶剪切生成不具膜滲透性的Fluo-3,從而滯留在胞內以發揮相應生理功能。

可見光激發Ca2+熒光探針的優勢:

與紫外光激發的熒光探針相比,如Fura-2Indo-1,可見光激發Ca2+探針具有以下特點:

1)適用于大多數的激光共聚焦測鈣系統,包括共聚焦激發掃描顯微鏡以及流式細胞儀等。

2 具有更強的染料吸收性能,使得更低濃度的探針即可成功檢測Ca2+變化,從而降低對活細胞的光毒性。

3Ca2+依賴性熒光強度增強,對Ca2+變化水平檢測敏感度更高。

4)降低樣品自熒光以及光散射的干擾。

5)兼容光激活探針以及UV-激發試劑,因此更方便于多參數檢測。

6)無光譜偏移。

Fluo-3, AM的應用

Fluo-31989年發現之后,其成像技術常用來揭示鈣離子信號傳遞內許多元素發展過程的空間動力學。還能被廣泛用在流式分析,比如籠鎖Ca2+螯合劑光活化、第二信使、神經傳導物質研究以及細胞水平的藥物篩選實驗。

Fluo-3, AM的應用實例

Fig. 1, Ligand-induced changes in intracellular free [Ca2+]. Splenic B cells were incubated with 4μM Fluo-3,  AM at 5 x 106 cells/ml and then adjusted to 7.5 x 106 cells/ml in Hank's Balanced Salt Solution (HBSS) containing bovine serum albumin (BSA) for the assay. Anti-IgM (0.3 μM), SDF-1 (6 nM), LPA (1 μM), or vehicle was added at time 0. The assay was performed in a 96-well plate using a Fluoroskan Ascent Microplate Fluorometer.   Measurements were recorded for 10 min followed by sequential addition of NP-40 (0.5% final) and EGTA (45mM final) to obtain fluorescence maximum and minimum values, respectively. Intracellular free [Ca2+] was calculated using the equation: [free Ca2+] = Kd (F - Fmin)/(Fmax - F). The Kd for Fluo-3 was assumed to be 390 nM. Data was corrected for baseline drift caused by leakage of Fluo-3 AM from cells into Ca2+-containing medium.   Each graph shows tracings of three replicate samples from one experiment. Experiments were performed at least five times.

Fig. 2, Metal-ion response screening for fluo-3. The maximum relative fluorescence intensity was measured for identical indicator concentrations in solutions containing 10 mM EGTA + 10 μM TPEN, 1 μM ion (100 μM for Mg2+) and 100 μM ion (10 mM for Mg2+). Results are plotted as fluorescence changes relative to the ion-free (10 mM EGTA + 10 μM TPEN) reference solution expressed as (F-Fo)/Fo, where F is the fluorescence intensity of ion-containing solutions and Fo is the fluorescence intensity of the reference solution. Blue bars indicate the response to 1 μM ion (100 μM for Mg2+), and red bars indicate the response to 100 μM ion (10 mM for Mg2+).

產品訂購【進口原料,現貨或1周貨期】

貨號

產品名稱

Ex/Em(nm) DNA-bound

規格

價格(元)

MX4503-50UG

Fluo-3, AM, Cell Permeant

鈣離子熒光探針,超級純

506/526

50μg

575

MX4503-250UG

506/526

5×50μg

2245

相關產品【進口原料,現貨或1周貨期】

貨號

產品名稱

Ex/Em(nm) DNA-bound

規格

價格(元)

MX4503-50UG

Fluo-3, AM, Cell Permeant

鈣離子熒光探針,超級純

506/526

50μg

575

MX4503-250UG

506/526

5×50μg

2245

MX4504-50UG

Fluo-4, AM, Cell Permeant

鈣離子熒光探針,超級純

494/516

50μg

375

MX4504-250UG

494/516

5×50μg

1500

MX4505-50UG

Fluo-8 AM, Cell Permeant

鈣離子熒光探針,超級純

490/514

50μg

MX4505-250UG

490/514

5×50μg

MX4507-50UG

Rhod-2, AM, Cell Permeant

鈣離子熒光探針,超級純

549/578

50μg

375

MX4507-250UG

549/578

5×50μg

1500

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作,主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承以人為本,以誠為信、合同守信的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

K
電話:021-54736159    傳真:021-54736159    手機:18121428569
郵箱: sales@maokangbio.com
地址:上海市徐匯區龍吳路2888弄23號902室    
版權所有:上海懋康生物科技有限公司
亚洲欧美天堂中文字幕,av男人的天堂在线观看第二区,女人天堂av在线视频免费