亚洲欧美天堂中文字幕
<sup id="oiuk6"></sup>
<rt id="oiuk6"></rt>
收藏本站    您好,歡迎光臨上海懋康生物科技有限公司!
登錄  |   注冊
當前位置: 首頁> 產品中心> 信號轉導研究相關 > 泛素-蛋白酶體途徑 > MG-132 蛋白酶體抑制劑
MG-132 蛋白酶體抑制劑
目錄號 MZ1701-10MG 售價 750.00元
規格 10mg 運輸溫度 室溫
其他名稱 MG132; MG 132; Z-Leu-Leu-Leu-CHO; Z-Leu-Leu-Leu-al; Z-LLL-CHO; Carbobenzoxy-L-leucyl- L-leucyl-L-leu 保存溫度 -20℃干燥保存
CAS號 133407-82-6 有效期 2年
應用 蛋白酶體Proteasome抑制劑,鈣蛋白酶Calpain抑制劑 訂購數量
產品簡介:

MG-132 蛋白酶體抑制劑


產品標簽

MG-132(Z-Leu-Leu-Leu-CHO);Proteasome Inhibitor蛋白酶體抑制劑;Calpain Inhibitor鈣蛋白酶抑制劑;;NF-κB activation;CAS:133407-82-6;


產品信息

產品名稱                         

產品編號               

CAS NO.          

規格           

價格(元)   

MG-132蛋白酶體抑制劑

MZ1701-0001MG

133407-82-6

1mg

215


產品描述

MG-132(MG132,MG 132),也稱為Z-Leu-Leu-Leu-al,Z-Leu-Leu-Leu-CHO,是一種強效的,可逆且具細胞滲透性的蛋白酶體Proteasome抑制劑(IC50=100 nM),還是細胞周期和細胞周期蛋白降解所需的鈣蛋白酶Calpain抑制劑(IC50=1.2 μM)[1]。A549細胞中MG-132(10μM)有效抑制TNFα誘導的NF-κB活化、IL-8基因轉錄和IL-8蛋白分泌,通過抑制蛋白酶體介導的IκBα降解來發揮作用[2]。MG-132(10μM)對NF-κB的抑制效應促使人胰腺癌細胞對依托泊甙Etoposide或阿霉素Doxorubicin的凋亡誘導更加敏感[3]。在PC3和DU145雄激素非依賴的前列腺癌(AIPCa)細胞系中MG-132能顯著改善六磷酸肌醇(IP6)對細胞代謝的減低能力[4]。


本品以凍干粉形式提供,純度≥98%,可溶于DMSO或無水乙醇配制成儲存液,之后用培養基或生理緩沖液稀釋到需要的工作濃度即可。


產品特性

1)CAS NO:133407-82-6

2)化學名:N-[(phenylmethoxy)carbonyl]-L-leucyl-N-[(1S)-1-formyl-3-methylbutyl]-L-leucinamide

3)同義名:MG132; MG 132; Z-Leu-Leu-Leu-CHO; Z-Leu-Leu-Leu-al; Z-LLL-CHO;Carbobenzoxy-L-leucyl- L-leucyl-L-leucinal;

4)分子式:C26H41N3O5

5)分子量:475.6

6)純度:≥98%

7)  外觀:白色至類白色固體

8)溶解性:溶于DMSO(30 mg/ml),無水乙醇(20mg/ml)

9)化學結構圖: 


保存與運輸方法

保存:-20℃干燥保存,至少2年有效。

運輸:常溫運輸。


產品使用

1) 使用前置于室溫回溫至少20min,并經短暫離心使得粉末/固體落在管底后再溶解。

2) 稱取適量粉末溶于無水DMSO配制儲存液(比如20 mg/ml,取500μl DMSO 加入10mg MG-132,渦旋混勻,直至完全溶解)。按照單次用量分裝后-20oC保存,避免反復凍存,至少1個月穩定, 也可置于-80℃延長保存周期。

3) 根據具體實驗應用,選擇合適的溶劑稀釋到所需濃度。體外細胞實驗,常用的工作濃度是5-50 μM,處理時間1-24h。本品的具體工作濃度和處理時間請參考相關文獻,并根據自身實驗條件(如實驗目的,細胞種類,培養特性等)進行摸索和優化。


文獻使用數據【僅作參考】

文獻1,MacLaren AP et al. p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells. Cell Death Differ. 2001 Mar;8(3):210-8. PMID: 11319603

體外研究:

細胞類型(Cell type):HeLa cells exposed to 10 or 100 J/m2 UV (254 nm) using a UV crosslinker.

藥物配制(Preparation):MG132 dissolved in DMSO and diluted in DMEM.

實驗方法(Assay):HeLa cells were grown to about 60% confluence in DMEM at 37°C in the presence of 5% CO2. MG132 dissolved in DMSO was added (5 μM final concentration). For mock treatment, DMSO alone was added. Cells were incubated for 1 h before UV irradiation. For UV treatment, medium in the Petri dishes was removed, and cells were exposed to 10 or 100 J/m2 UV (254 nm) using a UV crosslinker. Medium containing MG132 or DMSO alone was added back to the respective Petri dishes, and cells were transferred back to the incubator for the time course experiment.

文獻2,Inoue S et al.The effect of proteasome inhibitor MG132 on experimental in?ammatory bowel disease. Clin Exp Immunol. 2009 Apr;156(1):172-82. PMID: 19220323

體內研究:

動物模型(Animal Model):Experimental murine colitis models: IL-10-deficient (IL-10?/?) mice and dextran sulphate sodium (DSS)-induced colitis

藥物配制(Preparation):MG132 was dissolved in dimethyl sulphoxide (DMSO) and then diluted in 500 μl sterile phosphate-buffered saline (PBS) for injection.

注射劑量(Dosages):Female IL-10?/? mice at 4 weeks of age were divided into four groups and treated with intraperitoneal injection three times a week as follows: group A, 0·01% DMSO as the control; group B, 0·6 μmol/kg MG132; group C, 3·0 μmol/kg MG132; and group D, 15·0 μmol/kg MG132. All mice were killed after 4 weeks of treatment by cervical dislocation under ether anaesthesia.

Female C57BL/6 mice were given 3% DSS (molecular weight 36–50 kDa) in their drinking water for 5 days, and then switched to regular drinking water. Mice were injected intraperitoneally with 15·0 μmol/kg MG132 (prepared in the same way as for IL-10?/? mice) or 0·01% DMSO as the control three times a week from day 0 to the end of the experiment. On day 10, the mice were killed.

給藥途徑(Administration):Intraperitoneal (i.p.) injection



注意事項 

1)本品僅用作科研用途,不得用作臨床診斷或治療,不得用于食品或藥品,絕對禁止用在人身上。

2)為了您的安全和健康,請穿實驗服并戴一次性手套操作。


參考文獻

[1] Tsubuki S, et al. Differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-leucine and tri-leucine. J Biochem,119(3), 572-576 (1996).

[2] Fiedler MA, et al. Inhibition of TNF-alpha-induced NF-kappaB activation and IL-8 release in A549 cells with the proteasome inhibitor MG-132. Am J Respir Cell Mol Biol, 19(2), 259-268 (1998).

[3] A. Arlt, J. Vorndamm, M. Breitenbroich, et al. Inhibition of NF- k B sensitizes human pancreatic carcinoma cells to apoptosis induced by etoposide (VP16) or doxorubicin. Oncogene 20, 859-868 (2001).

[4] Diallo JS, et al. Enhanced killing of androgen-independent prostate cancer cells using inositol hexakisphosphate in combination with proteasome inhibitors. Br J Cancer, 99(10), 1613-1622 (2008).



相關產品

貨號

名稱

規格          

MZ0201-10MG          

Rapamycin (Sirolimus) 雷帕霉素(西羅莫司)

10mg             

MZ0342-5MG

Dorsomorphin Dihydrochloride

5mg

MX3270-1MG

Pan-caspase Inhibitor Z-VAD(OMe)-FMK (Powder)      

1mg

MZ3403-1G

Metformin HCl 鹽酸二甲雙胍

1g

MZ1701-1MG

MG-132 蛋白酶體抑制劑

1mg

MZ1704-10MG

Bortezomib (PS-341)  硼替佐米

10mg



 

 — — Written/Edited by V. Shallan【版權歸MKBio懋康所有】

 

 

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作,主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本,以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。


K
電話:021-54736159    傳真:021-54736159    手機:18121428569
郵箱: sales@maokangbio.com
地址:上海市徐匯區龍吳路2888弄23號902室    
版權所有:上海懋康生物科技有限公司
亚洲欧美天堂中文字幕,av男人的天堂在线观看第二区,女人天堂av在线视频免费